Preparative Electrophoresis: An Improved Method for the Isolation of Human Recombinant Apolipoprotein A-I
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چکیده
منابع مشابه
Preparative electrophoresis: an improved method for the isolation of human recombinant apolipoprotein A-I.
Protein expression is a powerful technique to study the functional role of proteins both in vivo and in vitro. Various systems have been established to produce proteins in milligram quantities; however, the purification of many expressed proteins commonly results in trace amounts. We report on an improved method for the isolation of apolipoprotein A-I (apoA-I) using the Model 491 Prep Cell (Bio...
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Apolipoprotein (apo) A-I is the major protein component of high-density lipoprotein (HDL) and plays key roles in the Reverse Cholesterol Transport pathway. In the past decade, reconstituted HDL (rHDL) has been employed as a therapeutic agent for treatment of atherosclerosis. The ability of rHDL to promote cholesterol efflux from peripheral cells has been documented to reduce the size of atheros...
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A method of preparative electrophoresis is described. A volume of approximately 1 ml. of protein solution can be applied with the cell used. A “marker” solution, applied adjacent to the sample to be separated, indicates the positions of the protein bands during the migration. The procedure has the advantages of a high degree of recovery, simplicity of operation, and utilization of an electropho...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1997
ISSN: 0736-6205,1940-9818
DOI: 10.2144/97235bm01